elisa kit  (Cusabio)

Journal: bioRxiv

Article Title: Late-Onset Preeclampsia is characterised by Accelerated Placental Aging

doi: 10.1101/2025.11.12.687933

Figure Lengend Snippet: a , Workflow for sample collection, trophoblast stem cell isolation, organoid formation and antioxidant treatment with superoxide dismutase (SOD). b , Schematic of SOD catalysing the dismutation of superoxide anions into oxygen. c , Telomere length dynamics over 4 weeks in culture (left) and violin plots of telomere length at endpoint (right) in control and LOPE organoids with or without SOD treatment. d , Representative immunofluorescence images of control and LOPE trophoblast organoids stained for γH2AX (DNA damage, green), 8-OHdG (oxidative stress, magenta) and DAPI (nuclei, blue) in the presence or absence of SOD. Scale bar, 100 µm. e, Quantification of 8-OHdG and f, γH2AX fluorescence intensity in control and LOPE trophoblast organoids with or without SOD treatment. Data presented as Tukey’s box and whisker plots. g , Representative immunohistochemistry images showing SA-β-Gal (violet) to assess senescence, co-stained with pan-cytokeratin to image trophoblast cells and counterstained with DAPI for nuclei; imaged at 40× magnification, and h, quantification of SA-β-Gal-positive staining. i-k, Concentrations of angiogenic factors in culture media after 4 weeks, including i, sFlt-1 (pg/mL), j, PlGF (pg/mL), and k, sFlt-1/PlGF ratio, in control and LOPE trophoblast organoids with or without SOD treatment. Data are presented as mean ± s.d. Trophoblast organoids were generated from n = 3 separate biological samples per fetal sex, per group; experiments performed in technical triplicate. For c , e - f , h , i-k, statistical analysis was performed using Welch’s ANOVA with multiple comparisons. **** p < 0.0001; ns, not significant. Source data file is provided.

Article Snippet: Dosage was as per Perera, et al. Superoxide dismutase (SOD, 30 U/mL; Active Recombinant Human Superoxide Dismutase #SOD1-8H, Creative Biomart, New York, USA).

Techniques: Cell Isolation, Control, Immunofluorescence, Staining, Fluorescence, Whisker Assay, Immunohistochemistry, Generated